whilst the other appears5invarient between Brassica lines containing different S alleles (by comparison with published sequence date). The organisation of genomic sequences homologous to both classes of S like cDNAs was investigated by Southern blotting using genomic DNA extracts from Brassica lines containing a range of S alleles. The expression of genes homologous to the two cDNAs has been investigated by Northern hybridization and found to be specific to stigma tissue; no transcripts of S like sequences are detectable in anther tissue over the developmental period during which expression of male S gene sequences might be expected.
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