it was necessary to develop a suitable means of discriminating between Pisum legumin and the endogenous protein. Cross reacting antibodies were precipitated out of a polyclonal antiserum by pre treatment with an extract from non transformed Nicotiana plumbaginifolia seeds and the remaining antibodies were then used to achieve specific localization of Pisum legumin. A method was also developed for the localization of the endogenous legumin like protein in the non transformed seeds
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had similar profiles and their constituent nucleic acids were degraded
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