although the breaking of the intimate association between the collagen molecules and the bone apatite crystallites exposes the crystallites to potential dissolution and recrystallization by percolating ground water. Disruption of the collagen apatite bond has been recognised in optical microscopy of thin sections by loss of the characteristic birefringence seen in unaltered bone when viewed in polarised light. The birefringence in histologically normal bone results from the strongly anisotropic orientation of the bone mineral crystallites imposed by their association with the highly organised collagen fibrils.
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