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containing the Nos NPT dominant selectable marker for expression in plants which was capable of being mobilised to Agrobacterium tumefaciens and forming cointegrates with pGV3850 and pGV3851. The frequencies of cointegrate formation of these plasmids were determined and the cointegrate structure established by Southern blotting. Inoculation of Kalanchoe diaigremontiana leaves and Nicotiana tabacum stems in vivo showed that pGV3851 is only weakly oncogenic. They may become more excitable or irritable or depressed. Others may detect a sensation
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